Part:BBa_I751100

luxR-Tokyo standard cloning site-GFP (J54140 delPr)

promoter-RBS-luxR-terminator-Tokyo standard cloning site-RBS-GFP By introducing a candidate promoter part into Tokyo standard cloning site, you can evaluate LuxR and AHL responsible activity of the candidate promoter part.

This part is made by deleting R0062 promoter from J54140.

This deletion allowed us to make full use of Tokyo Standard (J54033 and J54025).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1130
  Illegal BamHI site found at 1118
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1822
  

Functional Parameters: Austin_UTexas

BBa_I751100 parameters

Burden Imposed by this Part:

Burden Value: -1.9 ± 4.5%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.